Glyceraldehyde Phosphate Dehydrogenase Activity with Triphosphopyridine Nucleotide and with Diphosphopyridine Nucleotide
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Glyceraldehyde phosphate dehydrogenase activity with triphosphopyridine nucleotide and with diphosphopyridine nucleotide.
The results presented here are part of a study of pentose synthesis in Alcaligenes fuecalis. The mechanism of pentose synthesis is of interest since the organism can synthesize pentose from acetate, but cannot use hexoses for growth (1). In the course of the investigation into the enzymes associated with this synthesis, glyceraldehyde phosphate dehydrogenase activity, with either triphosphopyri...
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In continuation of our investigation of liver diphosphopyridine nucleotide-linked aldehyde dehydrogenase (1)) we have undertaken a study of various classes of inhibitors of the enzyme. Interest in aldehyde oxidation has been increasing in recent years from several points of view. The classical problem of ethanol oxidation in viva still has many unresolved facets (Z), and the enzymes responsible...
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Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) mRNA level quantitation is widely used as an invariant control for gene expression analysis. Only three vertebrate GAPDH cDNAs have been hitherto isolated: human (1), rat (2) and chick (3). In order to get a probe for RNAse-mapping experiments on hamster {Cricetulus griseus) mRNAs, we constructed a cDNA library in pBluescript KSH (St...
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Estabrook, R. W. (1955). Fed. Proc. 14, 45. Goddard, D. R. (1944). Amer. J. Bot. 31, 270. Hill, R. (1954). Nature, Lond., 174, 501. Hill, R. & Bhagvat, K. (1939). Nature, Lond., 143, 726. Hill, R. & Hartree, E. F. (1953). Annu. Rev. Pl. Physiol. 4, 115. Hill, R. & Scarisbrick, R. (1951). New Phytol. 50, 98. Holton, F. A. (1955). Biochem. J. 61, 46. Keilin, D. (1925). Proc. Roy. Soc. B, 98, 312....
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The dismutation of acetaldehyde to ethyl alcohol and acetic acid has been shown to occur in animal tissues (1, 2). The dependence of this reaction on diphosphopyridine nucleotide was demonstrated by von Euler and Brunius (3). Partial purification of the responsible enzyme system was achieved by Dixon and Lutwak-Mann (4). These authors clearly demonstrated that the mutase activity was independen...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1959
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)69832-1